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Flow Cytometer BioCyte-B5R3 BioCyte-B5R3V7Clinical & Analytical

BB-158785838

Flow Cytometer BioCyte-B5R3 BioCyte-B5R3V7

Introduction: Flow cytometer is a high-precision analytical instrument widely used in life sciences, medicine, immunology, and other fields. It integrates fluid mechanics, optics, electronics, and computer technology to rapidly analyze and sort individual cells (or particles) in a liquid suspension. By detecting scattered light signals and fluorescent signals from cells labeled with specific probes, the instrument can quantitatively measure multiple physical and chemical properties of cells (such as size, granularity, surface antigens, and intracellular components) at a speed of thousands to tens of thousands of cells per second, enabling high-throughput, multi-parameter, and non-destructive cellular analysis.

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Details

Feature s : Configuration U pgrade Up to 3 lasers and 15 fluorescent channels are configured, with a maximum of 18 detection parameters for BioCyte-B5R3V7 Powerful F unction High resolution, high sensitivity and high stability Automatic Full-automatic detection process, synchronous collection/analysis Automatic A nalysis Automatic fluorescence compensation adjustment function based on database, without changing the acquisition conditions Absolute C ount ing The absolute counting method based on the volume method of plunger pump is more accurate Injection O ptions The autosampler supports 40-tube Rotary/96-well microplate, which is simple and efficient High sensitivity and high resolution Fluorescence sensitivity of Rainbow calibration particles(8 peaks) Resolution of Rainbow fluorescent particles BioCyte D esign I nnovation New WDM splitter module The new WDM splitter module not only reduces the number of filters used but also reduces energy loss, further improving the sensitivity of fluorescence detection. New autosampler with mixing function Highly efficient and time-saving, compatible with 40-tube rotary/96-well microplate autosampler Software Features Chinese and English software, easy to learn and operate, can be switched freely Modular design, fully automatic detection, simultaneous acquisition and analysis One-click import of templates, custom editing reports based on needs Automatic fluorescence compensation adjustment function, no need to change acquisition conditions BioCyte Flow Cytometer Main applications Oncology and Hematology • Analysis of 6-color human peripheral blood immune cell subsets • Analysis of 9-color human peripheral blood immune cell subsets • Detection of dendritic cells (DC) • Phenotypic characterization of natural killer cell(NK) populations • Characterization of PBMC T cell receptors • Detection of CD62P Platelet Activation Immunology • Detection of T cell proliferation(CFSE) • Detection of B-cell antibody class • NK cell-mediated clearance of spleen cells • Detection of Treg cell subsets Stem C ells • Flow cytometry analysis of hematopoietic stem cells from mouse bone marrow • Detection of CAR-T extracellular tumor killing • Analysis of mesenchymal stem cells from bone marrow and endometrium of mice • Flow cytometry analysis of mouse hematopoietic progenitor cells Cell B iology • Cell activity test • Cell cycle detection • Cell proliferation detection • Cell apoptosis detection Oceanography • Detection of quantity, size and morphology of marine microorganisms • Detection of DNA content in marine microorganisms • Determination of Liposome Content in Marine Microalgae • Detection of marine bacterial activity and membrane integrity Environmental M onitoring • Flow cytometric detection of bacterial life and death • Absolute count of virus, bacteria and algae • Detection of bacterial periodic ploidy • Algae classification and counting Clinical • Lymphocyte subgroup analysis • HLA-B27 detection • Monocyte HLA-DR detection • Detection of neutrophil CD64 • Regulatory T cell detection • T cell activation and diseases • Th1 Th2 detection • Th17 cell detection • Blocking Antibody Detection • Cytokine detection • Platelet-leukocyte adhesion • Platelet autoantibody detection • Platelet activation and diseases • Paroxysmal nocturnal hemoglobinuria • Leukemia immunotyping test • Detection of residual focus of leukemia • MDS immune typing • Hematopoietic stem cell count • Lymphoma immunotyping • Detection of cell cycle and ploidy Flow Cytometry Laser/Channel/Fluorochrome Configuration Table Laser Wavelength Channel Filter Recommended Fluorescent Dyes BioCyte-B5R3V7 BioCyte-B5R3 488nm BL1 525/40 FITC, EGFP、BB515, Alexa Fluor 488 ✓ ✓ BL2 575/30 PE, DsRed ✓ ✓ BL3 620/30 PE-Texas Red, ECD, PE-CF594, PI, 7-AAD ✓ ✓ BL4 697/60 PerCP, PE-Cy5, PerCP-Cy5.5, PE-Cy5.5, BB700, PerCP-eFluor710 ✓ ✓ BL5 780/60 PE-Cy7, PE-Alexa Fluor 750 ✓ ✓ 638nm RL1 670/20 APC, Cy5, Alexa Fluor 647 ✓ ✓ RL2 720/40 APC-R700, Alexa Fluor 680, Alexa Fluor 700 ✓ ✓ RL3 780/60 APC-Cy7, APC-eFluor780, APC-H7 ✓ ✓ 405nm VL1 425/20 BV421 ✓ VL2 460/40 Pacific Blue, V450, BV480 ✓ VL3 525/40 BV51, V500 ✓ VL4 605/40 BV605 ✓ VL5 660/40 BV650 ✓ VL6 720/40 BV711 ✓ VL7 780/60 BV786, BV750 ✓ Technical Parameters: Model BioCyte-B5R3 BioCyte-B5R3V7 Software English/Chinese PC CPU i5/16G DDR4/1T hard disk+27 inches monitor Power Supply AC220/110V±10%, 50/60Hz External Size(W*D*H) 530*450*470mm Net Weight 35kg Packing Size(W*D*H) Main Instrument: 860*575*720mm Accessories: 860*680*810mm Reagent: 380*280*320mm Gross Weight Main Instrument: 67kg Accessories: 53.5kg Reagent: 16kg Recommended Reagent Information: Item Recommended Reagent Application Department Lymphocyte Subset T Analysis CD45 CD4 CD3 CD8 Clinical Laboratory Infectious Disease Department Oncology Department Department of Rheumatology and Immunology Department of Pediatrics Blood Specialty Lymphocyte Subset T/B/NK Analysis CD45 CD3 CD16 CD56 CD19 Absolute Count of CD4+T Lymphocyte CD45 CD4 CD3 CD8 CDC (AIDS) Infectious Disease Department Cytokine Th1/Th2/Th17 Analysis Th1: IL-2, IFN-γ, TNF-α Th2: IL-4, IL-6, IL-10 Th17: IL-17 Infectious Disease Department Oncology Department Department of Rheumatology and Immunology Department of Organ Transplantation Respiratory Department HLA-B27 Detection for AS HLA-B27-FITC HLA-B7-PE IgG2a-FITC IgG1-PE Department of Rheumatology and Immunology Orthopedics DFI Analysis Acridine orange (AO) Staining Solution Reproductive Department Infertility Department Reproductive Medicine Centre