Fluorescent Quantitative PCR Detection System

Details

Feature: ♦ Six independent temperature control ♦ Thermal compensation technology of temperature module ♦ No edge effect of temperature and optics have ♦ Maintenance-free long - life continuous spectral excitation source ♦ Synchronous and fast acquisition of low temperature CCD 0.15s in cold state ♦ Relative quantitative high sensitivity 1.5 times effective discrimination ♦ Flexible, open and user-friendly software platform ♦ Open reagent consumable platform Application: ♦ Basic materials research ♦ Pathogen detection ♦ Public health safety surveillance ♦ Meat products adulterated ♦ Transgenic testing ♦ Food safety inspection ♦ Drug development and rational drug use ♦ Precise tumor treatment ♦ Gene expression ♦ Genetic screening ♦ Genotyping ♦ Stem cell research Temperature control module On the basis of the six independent temperature control technology, using the enclosed liquid heat transfer efficiency, combined with environmental scanning monitoring regulation auxiliary infrared with heating and intelligent variable frequency system voltage, current, ensures the module heating speed, effectively prevents temperature overshoot no edge effect and the evaporation temperature, not only saves your precious time to amplify the required temperature uniformity and the repeatability of the results achieved perfection, no matter what kind of test mode, application, it can get good data uniformity and repeatability. Optical module On behalf of the world's leading optical transmission and collection technology, use optical fiber after the excitation light source for reaction system and the emission of light from the reaction system after excitation, the energy attenuation without conduction to each reaction holes and cold CCD, from physical properties to ensure consistency and authenticity of the excitation and detection, and greatly improve the detection sensitivity, can make your low effective detection and distinguish easily copy sample. The channel mismatch function is added to extend the application field of qPCR to the protein level and provide a new way for the construction of multiple systems of diagnostic reagents. Channel Excitation wavelength (nm) Emission wavelength (nm) Examples of fluorescent dyes 1 Blue 460-480 512-528 FAM/SYBR Green I/Eva Green… 2 Green 515-535 562~578 VIC/JOE/HEX/TET… 3 Yellow 560~580 612~628 ROX/Texas Red … 4 Red 610-630 662~678 Cy5… 5 Red 660-680 702~718 Cy5.5/Quasar705… ♦ Channel combination: 6-color excitation light filter and 6-color detection light filter can detect≥21 different fluorescence spectra, channels are completely open, supporting third-party reagent optimization Support FRET application. ♦ Multichannel static fluorescence function: analysis of starting template and end product. ♦ Filter can be customized according to customer need Software platform Real-time monitoring, automatic discrimination and calculation of positive and negative results, automatic establishment of standard curve, absolute/relative quantitative, multiple quantitative, fusion curve, gene mutation, quality control graphic analysis, fluorescence correction, PCR amplification efficiency, high-resolution fusion curve (optional match), etc. Models： Technical parameters: Product Name Fluorescence Quantitative PCR Detection System Model MA-6000 Sample Capacity 96x0.2ml PCR plate, 12x8-strip tubes, 96*0.2ml single tube Reaction System 6~125μl Dynamics Range 1-1010 copies Channel 5 Emission Light Full-wavelength maintenance-free halogen tungsten lamp Detector -20℃ CCD Detection Path F1 F2 F3 F4 F5 Suitable Probe/Dye FAM/SYBR Green/Eva Green/LC Green/Fluorescein VIC/HEX/TET/Cy3/JOE/Alex a555 ROX/Cy3.5/Texas Red Cy5/LC Red640 Cy5.5/LC Red705 Excitation Wavelength 380~780nm Detection Wavelength 380~780nm Fluorescence Detection Repeatability CV≤3% Fluorescence Detection Precision CV≤ 5% Sample Detection Repeatability CV≤3% Fluorescence Detection Linearity r≥0.980 Confidence It can effectively distinguish 5000 and 1000copies with99.8% confidence degree Module Temp. Range 4-100℃(resolution:0.1℃) Heating Speed 5.0℃/s(Max), 3.5℃/ s(Avg) Cooling Speed 5.0℃/s(Max), 3.2℃/ s(Avg) Temp. Accuracy ±0.1℃ Temp. Uniformity ≤ ±0.25℃ Number of Temp. Control Areas 6 Gradient Temp. Range 1-32℃ Hot-Lid Temp. Range 104℃, Automatic Hot-lid Special Function Absolute quantification, Relative Quantification, Genotyping, Amplification efficiency calculation, Melting curve. Operation System Windows 7 64-bit or higher Power Supply 100~240V,50~60Hz Dimension(L*W*H) mm 600*390 *320 Port Method USB Port Packing Size(L*W*H) Mm 850*600 *650 Gross Weight 51